Composite

Part:BBa_K2957036:Design

Designed by: Ethan Levy   Group: iGEM19_MIT   (2019-10-20)


hEF1a, synCD19


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1525
    Illegal SpeI site found at 92
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 92
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1525
    Illegal SpeI site found at 92
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1525
    Illegal SpeI site found at 92
    Illegal AgeI site found at 1531
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1231


Design Notes

We needed to consider which cell type this plasmid would go into and the transfection efficiency was often dependent on promoter in the genetic circuit/construct.


Source

This part was from mentor Shiva Razavi, who gave us the part when we were interested in potentially pursuing the HL60 cell to HEK cell communication synNotch system as a proof of concept of chemotaxis.

References